plasmid cDNA encoding Rz-vMwt CG
10296 b
Seq: Oct 2006
Includes: vector, T7 promoter and ribozyme seq

plasmid cDNA encoding vMC0 CG
10906 b
Seq: Oct 2006
Includes: Includes: vector and T7 promoter

plasmid pF/R-wt
8686 b
Seq: Oct 2006, For pdf map, click here
Includes: CMV promoter, T7 promoter, Fluc gene, EMCV IRES (wt), Rluc gene, 3’ UTR, polyA site, and complete vector (derivative of CloneTech pIRES)

plasmid pF/L
7925 b
Seq: Oct 2006, For pdf map, click here
Includes: CMV promoter, T7 promoter, Fluc gene, EMCV IRES (wt), EMCV Leader gene, 3' UTR, polyA site, and complete vector (derivative of CloneTech pIRES)

plasmid pF/L-C19A
7925 b
Seq: Oct 2006
Includes: Same as pF/L except for L zinc finger mutation (C to A) at aa 19 of L seq

plasmid pF/L-delta-a
7856 b
Seq: Oct 2006
Includes: Same as pF/L except with deletion within L gene acidic domain (aa: 37-61 are missing)

EMCV Rueckert strain CG
7835 b (no 3' polyA included)
Seq update: May 2006
polyC: C125UCUC3UC10
GB: NC_001479 (also: M81861)

EMCV C9 recomb strain CG
7722 b (including 3' polyA of 10 b)
Seq: Dec 2005
polyC: C9
GB: D0288856

Mengo M strain CG
7761 b (no 3' polyA included)
Seq: Dec 2005 (rev)
polyC: C44UC10
GB: L22089

Mengo M derivative recomb strain CG
7740 b (including 3' polyA of 20 b)
Seq: Dec 2005 (rev)
polyC: none

Mengo M derivative recomb strain CG
7765 b (including 3' polyA of 10 b)
Seq: Dec 2005 (rev)
polyC: C44UC10
GB: D0294633

Qiagen maxiprep for cDNAs,
T7 RNA transcription, RNA transfection of HeLa cells, virus infection of HeLa cells, relevant buffers and media

pFR or pFL plasmid growth protocol, plasmid isolation, transfection procedures, and luciferase assays.