Palmenberg lab: sequences, plasmids, protocols
Table updated: Sept 8. 2014

Plasmid Seqs

File format

Description/Information

pEC9

txt

plasmid cDNA encoding vEC9 CG
10889 b
Seq: Oct 2006
Includes: map with vector and T7 promoter
reference

pMwt

txt

plasmid cDNA encoding vMwt CG
10961 b
Seq: Oct 2006
Includes: map with vector and T7 promoter
reference

Rz-pMwt

txt

plasmid cDNA encoding Rz-vMwt CG
10296 b
Seq: Oct 2006
Includes: vector, T7 promoter and ribozyme seq
reference

pMC0

txt

plasmid cDNA encoding vMC0 CG
10906 b
Seq: Oct 2006
Includes: map with Includes: vector and T7 promoter
reference

pC15

txt

plasmid cDNA encoding HRV C15 CG
10345 b
Seq: Dec 2010, For pdf map, click here
Includes: Includes: vector and T7 promoter
.Seq in fasta format.
reference

pF/R-wt

txt

plasmid pF/R-wt
8665 b
Seq: Jan 2013, For pdf map, click here. For Lasergene (*.pdf) annotated map and seq click here.
Includes: CMV promoter, T7 promoter, Fluc gene, EMCV IRES (wt), Rluc gene, 3 UTR, polyA site, and complete vector (derivative of CloneTech pIRES). This seq and map were updated from previous Oct-'06 version of plasmid
reference

txt

plasmid pF/L
7925 b
Seq: Oct 2006, For pdf map, click here
Includes: CMV promoter, T7 promoter, Fluc gene, EMCV IRES (wt), EMCV Leader gene, 3' UTR, polyA site, and complete vector (derivative of CloneTech pIRES)
reference

txt

plasmid pF/L-C19A
7925 b
Seq: Oct 2006
Includes: Same as pF/L except for L zinc finger mutation (C to A) at aa 19 of L seq
reference

txt

plasmid pF/L-delta-a
7856 b
Seq: Oct 2006
Includes: Same as pF/L except with deletion within L gene acidic domain (aa: 37-61 are missing)
reference

pHalo3xNLS txt

plasmid pHalo3XNLS
4936 b
Seq: Feb 2009
Includes: Halotag gene linked to 3xNLS under control of CMV promoter. Will express protein after eucaryotic cell transfection.
reference

pR16.11 txt

plasmid pR16.11
9875 b
Seq: Sept 2014, For pdf map, click here
Includes: infectious RV-A16

pWR3-26(B14) txt

plasmid pWR3-26(B14)
9490 b
Seq: Sept 2014, For pdf map, click here
Includes: infectious RV-B14

 

Virus Seqs

File format

Description/Information

vE-R

txt

EMCV Rueckert strain CG
7835 b (no 3' polyA included)
Seq update: May 2006
polyC: C125UCUC3UC10
GB: NC_001479 (also: M81861)

vEC9

txt

EMCV C9 recomb strain CG
7722 b (including 3' polyA of 10 b)
Seq: Dec 2005
polyC: C9
GB: D0288856

vM-M

txt

Mengo M strain CG
7761 b (no 3' polyA included)
Seq: Dec 2005 (rev)
polyC: C44UC10
GB: L22089

vMC0

txt

Mengo M derivative recomb strain CG
7740 b (including 3' polyA of 20 b)
Seq: Dec 2005 (rev)
polyC: none

vMwt

txt

same as Rz-vMwt

Rz-vMwt

txt

Mengo M derivative recomb strain CG
7765 b (including 3' polyA of 10 b)
Seq: Dec 2005 (rev)
polyC: C44UC10
GB: D0294633

 

Protocols

File format

Description/Information

virus & virus plasmids

pdf

Qiagen maxiprep for cDNAs,
T7 RNA transcription, RNA transfection of HeLa cells, virus infection of HeLa cells, relevant buffers and media

pdf

pFR or pFL plasmid growth protocol, plasmid isolation, transfection procedures, and luciferase assays.