Palmenberg lab: sequences, plasmids, protocols
Table updated: Jan 18, 2013 |
Plasmid Seqs |
File format |
Description/Information |
| pEC9 |
txt |
plasmid cDNA encoding vEC9 CG
10889 b
Seq: Oct 2006
Includes: vector and T7 promoter
reference
|
| pMwt |
txt |
plasmid cDNA encoding vMwt CG
10961 b
Seq: Oct 2006
Includes: vector and T7 promoter
reference
|
| Rz-pMwt |
txt |
plasmid cDNA encoding Rz-vMwt CG
10296 b
Seq: Oct 2006
Includes: vector, T7 promoter and ribozyme seq
reference
|
| pMC0 |
txt |
plasmid cDNA encoding vMC0 CG
10906 b
Seq: Oct 2006
Includes: Includes: vector and T7 promoter
reference
|
pC15 |
txt |
plasmid cDNA encoding HRV C15 CG
10345 b
Seq: Dec 2010, For pdf map, click here
Includes: Includes: vector and T7 promoter.Seq in fasta format.
reference
|
| pF/R-wt |
txt |
plasmid pF/R-wt
8665 b
Seq: Jan 2013, For pdf map, click here. For Lasergene (*.pdf) annotated map and seq click here.
Includes: CMV promoter, T7 promoter, Fluc gene, EMCV IRES (wt), Rluc gene, 3Õ UTR, polyA site, and complete vector (derivative of CloneTech pIRES). This seq and map were updated from previous Oct-'06 version of plasmid
reference
|
|
txt |
plasmid pF/L
7925 b
Seq: Oct 2006, For pdf map, click here
Includes: CMV promoter, T7 promoter, Fluc gene, EMCV IRES (wt), EMCV Leader gene, 3' UTR, polyA site, and complete vector (derivative of CloneTech pIRES)
reference
|
|
txt |
plasmid pF/L-C19A
7925 b
Seq: Oct 2006
Includes: Same as pF/L except for L zinc finger mutation (C to A) at aa 19 of L seq
reference
|
|
txt |
plasmid pF/L-delta-a
7856 b
Seq: Oct 2006
Includes: Same as pF/L except with deletion within L gene acidic domain (aa: 37-61 are missing)
reference
|
| pHalo3xNLS |
txt |
plasmid pHalo3XNLS
4936 b
Seq: Feb 2009
Includes: Halotag gene linked to 3xNLS under control of CMV promoter. Will express protein after eucaryotic cell transfection.
reference
|
| |
Virus Seqs |
File format |
Description/Information |
| vE-R |
txt |
EMCV Rueckert strain CG
7835 b (no 3' polyA included)
Seq update: May 2006
polyC: C125UCUC3UC10
GB: NC_001479 (also: M81861)
|
| vEC9 |
txt |
EMCV C9 recomb strain CG
7722 b (including 3' polyA of 10 b)
Seq: Dec 2005
polyC: C9
GB: D0288856
|
| vM-M |
txt |
Mengo M strain CG
7761 b (no 3' polyA included)
Seq: Dec 2005 (rev)
polyC: C44UC10
GB: L22089
|
| vMC0 |
txt |
Mengo M derivative recomb strain CG
7740 b (including 3' polyA of 20 b)
Seq: Dec 2005 (rev)
polyC: none
|
| vMwt |
txt |
same as Rz-vMwt
|
| Rz-vMwt |
txt |
Mengo M derivative recomb strain CG
7765 b (including 3' polyA of 10 b)
Seq: Dec 2005 (rev)
polyC: C44UC10
GB: D0294633
|
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Protocols |
File format |
Description/Information |
| virus & virus plasmids |
pdf |
Qiagen maxiprep for cDNAs,
T7 RNA transcription, RNA transfection of HeLa cells, virus infection of HeLa cells, relevant buffers and media
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pdf |
pFR or pFL plasmid growth protocol, plasmid isolation, transfection procedures, and luciferase assays.
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