Nature -Advance Online Publications

Nature Structural aamp; Molecular Biology - AOP - nature.com science feeds

An accurately preorganized IRES RNA structure enables eIF4G capture for initiation of viral translation The solution structure of the J-K region of the EMCV IRES establishes a preorganized recognition mode for hijacking translation initiation factor 4G. Structure of a pathogen effector reveals the enzymatic mechanism of a novel acetyltransferase family Structural and functional analyses of HopZ1a, a member of the YopJ family of bacterial type III–secreted effectors, reveal the structural basis of YopJ effectors’ noncanonical acetyltransferase activity and allosteric regulation by inositol hexakisphosphate. Cryomicroscopy provides structural snapshots of influenza virus membrane fusion Cryo-EM and tomography imaging of influenza virus fusion with target membranes reveal structural intermediates of HA surface glycoprotein and their interactions with membranes as well as ultrastructural changes in the virus that accompany membrane fusion. One-way membrane trafficking of SOS in receptor-triggered Ras activation Analyses in supported lipid bilayers and in cells shed light on the roles of multiple SOS-membrane interactions in SOS's membrane recruitment and association, processive activation of Ras and signal attenuation. Iterative structure-based improvement of a fusion-glycoprotein vaccine against RSV An interactive structure-based approach was used to improve a vaccine antigen against respiratory syncytium virus (RSV), thus leading to immunogens with higher stability that elicit higher neutralizing titers in mice. Clathrin-coat disassembly illuminates the mechanisms of Hsp70 force generation Light-scattering kinetics and atomic force and electron microscopy analyses show that Hsp70-mediated disassembly of clathrin cages occurs via a collision-pressure mechanism consistent with the entropic pulling model. Spiral architecture of the Hsp104 disaggregase reveals the basis for polypeptide translocation A cryo-EM structure of yeast AAA+ protein disaggregase Hsp104 with AMP-PNP reveals a spiral arrangement of the protomers and a continuous path for polypeptide translocation that explains Hsp104's processivity mechanism during disaggregation. m1A and m1G disrupt A-RNA structure through the intrinsic instability of Hoogsteen base pairs The inability of A-form RNA to form Hoogsteen base pairs provides a mechanism for how post-transcriptional modifications can disrupt RNA structure and might help explain why DNA is the molecular choice for storing genetic information. Structural basis for tRNA modification by Elp3 from Dehalococcoides mccartyi The eukaryotic Elongator complex participates in modification of uridines in tRNAs. Structural and functional work on a bacterial Elp3, the catalytic subunit of Elongator, provides insight into the function and mechanism of this important enzyme. Skp is a multivalent chaperone of outer-membrane proteins Mass spectrometry, kinetics studies and in silico analyses indicate that multiple copies of the Skp chaperone are required for sequestration of 16-stranded or larger OMPs and prevention of their aggregation. An atomic structure of the human 26S proteasome A cryo-EM structure of the human 26S proteasome in a resting state at an average resolution of 3.5Å reveals details in the interactions between subunits. An additional structure of the proteasome with USP14 bound suggests a mechanism for its activation. Extended surface for membrane association in Zika virus NS1 structure The crystal structure of full-length NS1 protein from Zika virus reveals an extended surface for membrane association and a highly variable polar surface. Structural basis of Cas3 inhibition by the bacteriophage protein AcrF3 The crystal structure of the phage anti-CRISPR protein AcrF3 in complex with Cas3 reveals its mode of inhibition of the CRISPR–Cas bacterial immune system.

Return To Top of the Page