|Nature Structural aamp; Molecular Biology - Issue - nature.com science feeds|
Structures of the human orexin receptor 1 (hOX1R) bound to a selective drug and the dual (hOX1R- and hOX2R-targeting) antagonist suvorexant reveal molecular mechanisms of selectivity in orexin-receptor subtypes.
Three recent reports explore how PRDM9 binds to meiotic hotspots within the genome and provide compelling evidence that hotspot erosion leads to speciation.
USP7 deubiquitinase is now shown to prevent ubiquitination of SUMO chains of replisome proteins, thereby regulating DNA replication-fork progression and origin firing.
NMR approaches are used to probe cotranslational folding of a nascent polypeptide with two domains in Escherichia coli. The work reveals that interactions with the ribosome inhibit acquisition of the native fold by the nascent chain.
New evidence that human telomerase RNA (hTR) degradation by EXOSC10 or DCP2 and XRN1 reduces telomerase activity when dyskerin is compromised suggests that RNA decay pathways may provide future therapeutic targets for telomere biology disorders.
Human orexin receptors (hOX1R and hOX2R) are GPCRs involved in sleep regulation. Structures of hOX1R bound to a selective antagonist or to a dual antagonist, functional assays and computational analyses reveal the basis for subtype selectivity.
Structural and cellular analyses reveal that the presence of an isoform-specificα-helix in myosin VI determines whether this motor protein functions in endocytosis or cell migration.
The crystal structure of human YL1, here established as an H2A.Z-deposition chaperone, in complex with an H2A.Z–H2B dimer reveals the molecular basis for the specificity of H2A.Z recognition.
The crystal structure of Drosophila melanogaster YL1 in complex with an H2A.Z–H2B dimer exposes a selective recognition mechanism distinct from those of other H2A.Z chaperones and suggests a hierarchical transfer mechanism mediating H2A.Z deposition.
Cancer-associated point mutations in the scaffold protein Axin derail Wnt signaling and promote tumor growth through formation of nonamyloid nanoaggregates that rewire the Axin interactome.
Monitoring ribosomal translocation from five structural perspectives with pre–steady state smFRET defines intramolecular conformational events within the EF-G–GDP–bound ribosome as rate-determining steps of directional substrate translocation.
Ensemble kinetics and FRET analyses reveal the sequence of collective motions on the E. coli ribosome during translocation promoted by EF-G and allow identification of a new early forward swiveling of the SSU head.
EaSeq, a user-friendly and freely available software tool, offers fast and comprehensive ChIP-sequencing data analyses, enabling experimentalists to easily extract information and generate hypotheses from genome-wide datasets.
|Nature Structural aamp; Molecular Biology - AOP - nature.com science feeds|
The crystal structure of the GP1–GP2 complex of the prototypical arenavirus LCMV in prefusion form sheds light on the conformational changes that the arenavirus glycoprotein undergoes to cause fusion.
Capsids from two herpesviruses (HSV-1 and PRV), imaged inside intact virions, are analyzed by cryo-EM. The maps allowed the construction of a complete model of subunit and domain organization, revealing extensive subunit contacts.
New data reveal that LINP1, a lncRNA overexpressed in triple-negative breast cancer, interacts with the Ku70–Ku80 complex and DNA-PKcs, thereby promoting NHEJ-mediated DNA double-strand-break repair.
Genetic ablation of EF4 in mice leads to male sterility due to mitochondrial translation defects, which can be compensated for in somatic tissues by mTOR-mediated upregulation of cytoplasmic translation.
Crystal structures of the human ADAR2 deaminase domain in complex with RNA duplexes reveal the mechanisms for ADAR2's action and explain its substrate preference. The work also provides a rationale to understand disease-related mutations.
High-resolution kinetic analysis and enzyme trapping assays reveal how PCNA coordinates 5′-flap generation and processing by Pol δ and FEN1 during Okazaki-fragment maturation.
B-cell translocation gene-4 (Btg4) bridges interactions of translation initiation factor eIF4E and CCR4–NOT deadenylase, thus triggering decay of maternal mRNA during mouse oocyte maturation.
SELEX selections and crystallographic analyses have allowed development of a DNA aptamer that inhibits autotaxin with high potency and specificity, and exhibits efficacy against bleomycin-induced pulmonary fibrosis in model mice.
The CARD-only protein INCA inhibits inflammasome assembly by capping caspase-1 CARD oligomers and preventing their further polymerization.
The sirtuin family protein SIRT6 maintains pericentric heterochromatin silencing at human centromeres through deacetylation of a newly discovered substrate, H3K18, thus protecting cells against mitotic errors, genomic instability and cellular senescence.
RNA interference constrains antisense lncRNA transcriptomes, and its loss during budding yeast evolution is associated with an increase in genome-wide expression of antisense lncRNAs.
Structural and functional analyses of a new family of tick-derived C5 inhibitors in complex with inhibitor OmCI and therapeutic antibody eculizumab reveal diverse mechanisms for inhibition and provide insight into C5 activation by C5 convertases.
α-synuclein amyloid fibrils are associated with Parkinson's disease. SSNMR analyses now reveal the atomic structure of a pathogenic human α-synuclein fibril, providing a framework for understanding fibril nucleation, propagation and interactions with small molecules.
The crystal structure of the C-terminal region of Zika virus nonstructural protein 1 (NS1) reveals a fold similar to those of other flaviviruses (dengue and West Nile viruses) but different surface electrostatic features.
An analysis of previously published data on fiber formation by sickle-cell hemoglobin reveals a universal curve when delay time is plotted against supersaturation (ratio of protein concentration to solubility).