Virus Imaging

Select by virus name
About Images
Art Gallery
Covers Gallery
ICTV 8th Color Plates
PS10 Screen Saver   

Virus Structure Tutorials

Triangulation Number
Topography Maps 3D

Virology Links

In the News

- News -
- Video -
- Blogs -
 * Virology Highlights
- Flu & H1N1 - (CDC|WHO)

Journal Contents

Nature Structural & Molecular Biology

Structure & Assembly (J.Virol)
Journal of Virology
J. General Virology
Virology Journal
Virus Genes

Educational Resouces

Video Lectures  NEW 
TextBook  NEW 
Educational Links
Educational Kids


Archived Web Papers

Jean-Yves Sgro
Inst. for Mol.Virology
731B Bock Labs
1525 Linden Drive Madison, WI 53706

Table of Contents for this page:

  • Current Issue
  • Advanced Online Publications Articles

  • Current Issue of Nature Structural and Molecular Biology

    Nature Structural aamp; Molecular Biology - Issue - science feeds

  • Revolution in the Polycomb hierarchy

  • Traditionally, maintenance of gene silencing by the Polycomb group proteins has been thought to involve recruitment of Polycomb repressive complex (PRC) 1 by PRC2-mediated trimethylation of K27 on histone H3. Three recent studies challenge this model by demonstrating that monoubiquitination of histone H2A, which is catalyzed by PRC1 complexes, can recruit PRC2 and potentiate its catalytic activity.

  • Channeling your inner energy

  • ATP is continuously synthesized inside mitochondria and exported to the cytoplasm via transporter and channel proteins residing in the inner and outer mitochondrial membranes, respectively. In this issue of Nature Structural aamp; Molecular Biology, a new crystal structure of the mitochondrial channel protein VDAC-1 provides the basis for a detailed simulation study that unravels the mechanism by which ATP diffuses across the outer mitochondrial membrane at a fast rate.

  • PAN-orama: three convergent views of a eukaryotic deadenylase

  • Post-transcriptional mRNA regulation is often attained by lengthening or shortening the 3′ poly(A) tail of a transcript. Eukaryotic mRNAs show a spectrum of deadenylation rates, thus allowing intricate control of gene expression, but the mechanisms that determine such rates are unclear. Three new studies highlight the structural and biochemical features of a key enzyme in removing poly(A) tails, the PAN2–PAN3 complex, providing clues to how different mRNA deadenylation rates can be achieved.

  • Molecular basis for pseudokinase-dependent autoinhibition of JAK2 tyrosine kinase

  • Cancer-associated mutations in the pseudokinase domain (JH2) of JAK2 lead to constitutive activation of its tandem kinase domain (JH1). Molecular dynamics simulations, supported by mutational analysis, provide a model for JH2-JH1 interactions that explains many of the JAK2-activating disease mutations.

  • Dicer-microRNA-Myc circuit promotes transcription of hundreds of long noncoding RNAs

  • Chang and colleagues report the involvement of a Dicer-microRNA-cMyc signaling axis in the transcriptional regulation of a large set of long noncoding RNAs (lncRNAs). These lncRNAs are specifically dependent on cMyc, as compared to divergently transcribed protein-coding genes.

  • The structure of the Pan2–Pan3 core complex reveals cross-talk between deadenylase and pseudokinase

  • Conti and colleagues present the crystal structure of the yeast deadenylase Pan2–Pan3 core complex, revealing a 1:2 stoichiometry and indicating how deadenylase and pseudokinase domains work together to promote RNA deadenylation.

  • An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to mediate mRNA deadenylation and decay

  • Structural analyses reveal the asymmetric assembly of Neurospora crassa PAN2–PAN3 complex and, along with functional work on the proteins from different species, indicate an essential role for PAN3 in coordinating PAN2-mediated deadenylation with subsequent steps in mRNA decay.

  • Nucleosome assembly is required for nuclear pore complex assembly in mouse zygotes

  • A new study using fertilized mouse eggs shows that nuclear pore complex (NPC) formation is dependent on nucleosome assembly. Preventing de novo histone deposition on sperm chromatin results in formation of a paternal nuclear envelope lacking NPCs, thus indicating a role for nucleosomes beyond DNA packaging.

  • Nucleosomal regulation of chromatin composition and nuclear assembly revealed by histone depletion

  • A new system to monitor the effects of nucleosome depletion in Xenopus egg extracts reveals that nucleosomes are required for spindle assembly and for recruitment of nuclear pore complex (NPC) components to the nuclear envelope for NPC formation.

  • Structure-guided simulations illuminate the mechanism of ATP transport through VDAC1

  • VDAC channels permeate metabolites from the mitochondrial intermembrane space to the cytosol. Markov state modeling, an approach used in protein-folding simulations, is now applied to examine ATP-permeation rates and pathways through mouse VDAC1.

  • Structural insights into the stabilization of MALAT1 noncoding RNA by a bipartite triple helix

  • The X-ray crystal structure and biochemical analysis of a triple helix formed between the expression and nuclear retention element (ENE) and the 3′ poly(A) tail of the human long noncoding RNA MALAT-1 reveals the basis of its stability and how it confers resistance to degradation.

  • The translational landscape of fission-yeast meiosis and sporulation

  • The first comprehensive analysis of the meiotic translational program of Schizosaccharomyces pombe by deep sequencing of ribosome-protected fragments identifies new translated sequences and highlights differences in the translational changes occurring during sexual differentiation of fission and budding yeasts.

    Return To Top of the Page

    Nature -Advance Online Publications

    Return To Top of the Page

    Nature Structural aamp; Molecular Biology - AOP - science feeds

  • Selectivity mechanism of a bacterial homolog of the human drug-peptide transporters PepT1 and PepT2

  • Guettou et al. describe structural studies on a bacterial homolog of PepT1 and PepT2 peptide transporters—nutrient transporters responsible for all peptide transport across the plasma membrane—in complex with three di- or tripeptides. The data suggest how the transporter's broad peptide specificity is achieved.

  • Structure of the mammalian 80S initiation complex with initiation factor 5B on HCV-IRES RNA

  • Eukaryotic initiation factor 5b (eIF5B) is essential for translation initiation. Spahn and colleagues now report cryo-EM structures of the mammalian 80S initiation complex associated with eIF5B that redefine eIF5B as a tRNA reorientation factor.

  • Identification of genes in toxicity pathways of trinucleotide-repeat RNA in C. elegans

  • A screen identifies 15 genes that modulate CUG-repeat toxicity in C. elegans, including those encoding RNA-export and clearance factors. Toxic RNAs are recognized by the NMD pathway via their 3' UTR GC content.

  • Allosteric enhancement of MAP kinase p38α's activity and substrate selectivity by docking interactions

  • MAP kinases recognize pathway-specific substrates via docking interactions. NMR analyses now reveal that docking interactions also stimulate ATP binding and phosphotransfer activity of p38α via an allosteric mechanism.

  • Structural basis for activity of highly efficient RNA mimics of green fluorescent protein

  • Structural elucidation of the RNA aptamer 'Spinach' reveals that a new G-quadruplex structure forms the fluorophore-binding site that confers the ability of the RNA to function as a GFP mimic.

  • Structural basis for RNA recognition in roquin-mediated post-transcriptional gene regulation

  • Roquin controls T-cell activity through interactions with mRNAs of stimulatory receptors. Structural and functional elucidation of its RNA-binding domain reveals how it interacts with constitutive decay elements in the 3' UTR of its targets to regulate their expression.

  • The ROQ domain of Roquin recognizes mRNA constitutive-decay element and double-stranded RNA

  • Roquin recognizes the CDE element in mRNAs to promote their decay. Crystal structures of human Roquin ROQ domain in complex with RNA reveals two distinct RNA-binding sites for stem-loop RNA and dsRNA.

  • Mechanism of asymmetric polymerase assembly at the eukaryotic replication fork

  • Eukaryotic DNA replication is carried out by two DNA polymerases, Polɛ and Pol δ. An in vitro–replication system reconstituted with purified yeast components identifies the factors that selectively recruit each polymerase for leading- or lagging-strand synthesis.

  • RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation

  • Mammalian RPRD proteins bind the phosphorylated CTD of RNA pol II with different affinities. Structural elucidation and characterization of their CTD interaction domains reveal the basis of RPRD binding preferences and a role in directing CTD dephosphorylation.

  • Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis

  • Plk4 regulates centriole duplication. Two centrosomal scaffold proteins, Cep192 and Cep152, are shown to interact with Plk4 in a temporally and spatially regulated manner, and structural analyses reveal that these interactions are mutually exclusive.

  • A protein-RNA specificity code enables targeted activation of an endogenous human transcript

  • A randomized RNA library is used to determine the specificities of RNA recognition by PUF repeats. The code is then used to design a protein that targets endogenous human cyclin B1 mRNA and activates its translation.

  • Delivering nonidentical twins

  • Two sibling DNA polymerases synthesize most of the eukaryotic nuclear genome. A new study provides insights into the distinct protein interactions that deliver these replicases for asymmetric leading- and lagging-strand replication and reveals possible cross-talk between DNA replication and other cellular processes.
    Return To Top of the Page